How should I choose PCR tubes/plates with different volumes?

Most of the volumes of PCR tubes can meet the requirements of PCR reactions. However, on the basis of meeting the experimental requirements, low-volume tubes are preferred. Because the low-volume reactor tubes/plates have less headroom, heat transfer is improved and evaporation is reduced. And when adding samples, it is necessary to avoid adding too much or too little. Too much will result in decreased thermal conductivity, spillage and cross-contamination, while adding too little may cause sample evaporation loss. You can choose a more suitable product according to the specific experimental requirements.

Common PCR tubes/plate specifications and volumes:

Single tube/tube strip: 0.5mL, 0.2mL, 0.15mL

96-well plate: 0.2mL, 0.15mL

384-well plate: 0.04mL